eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn2+Imaging
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چکیده
منابع مشابه
eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn Imaging
Zn plays essential and diverse roles in numerous cellular processes. To get a better understanding of intracellular Zn homeostasis and the putative signaling role of Zn, various fluorescent sensors have been developed that allow monitoring of Zn concentrations in single living cells in real time. Thus far, two families of genetically encoded FRET-based Zn sensors have been most widely applied, ...
متن کاملeZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn2+ Imaging
Zn(2+) plays essential and diverse roles in numerous cellular processes. To get a better understanding of intracellular Zn(2+) homeostasis and the putative signaling role of Zn(2+), various fluorescent sensors have been developed that allow monitoring of Zn(2+) concentrations in single living cells in real time. Thus far, two families of genetically encoded FRET-based Zn(2+) sensors have been m...
متن کاملMonitoring cytosolic and ER Zn(2+) in stimulated breast cancer cells using genetically encoded FRET sensors.
The Zn(2+)-specific ion channel ZIP7 has been implicated to play an important role in releasing Zn(2+) from the ER. External stimulation of breast cancer cells has been proposed to induce phosphorylation of ZIP7 by CK2α, resulting in ZIP7-mediated Zn(2+) release from the ER into the cytosol. Here, we examined whether changes in cytosolic and ER Zn(2+) concentrations can be detected upon such ex...
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Förster Resonance Energy Transfer (FRET) between two fluorescent proteins can be exploited to create fully genetically encoded and thus subcellularly targetable sensors. FRET sensors report changes in energy transfer between a donor and an acceptor fluorescent protein that occur when an attached sensor domain undergoes a change in conformation in response to ligand binding. The design of sensit...
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Title: 1 The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and 2 Molecular Effects on Central Neurons 3 4 Abbreviated Title: 5 Signaling mechanisms of PDF and sNPF 6 7 Author Names and Affiliations: 8 Christopher G. Vecsey, Nicolás Pírez, Leslie C. Griffith 9 10 National Center for Behavioral Genomics, Volen National Center for Complex Systems and 11 Department of Bio...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2015
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.5b00211